Improved Biomonitoring of Urban Freshwater Ecosystems Using DNA Barcodes

Grant number: LP150100876 | Funding period: 2015 - 2019

Completed

Abstract

This project aims to provide rapid diagnostic detection of environmental change in aquatic ecosystems at reduced cost. Next-generation sequencing (NGS) can transform monitoring of aquatic ecosystems by identifying many species in multiple biological samples. This project aims to use NGS technology to identify macroinvertebrate species as biological indicators in urban freshwater ecosystems to provide more rapid, sensitive and cost-effective monitoring compared to traditional methods. The project uses DNA barcodes that identify species to detect species in samples processed with NGS. The project also plans to use previously collected samples to explore species diversity and detect indicator s..

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Related publications (3)

Using unsorted sweep-net samples to rapidly assess macroinvertebrate biodiversity

ME Carew, RA Coleman, KL Robinson, AA Hoffmann

2021-09-01

Macroinvertebrate biodiversity is routinely used to assess the ecological condition of freshwater environments. Macroinvertebrates..

Can high-throughput sequencing detect macroinvertebrate diversity for routine monitoring of an urban river?

ME Carew, CR Kellar, VJ Pettigrove, AA Hoffmann

2018-02-01

Macroinvertebrate families identified through morphological examination have traditionally been used in routine assessment of fres..

Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?

ME Carew, RA Coleman, AA Hoffmann

2018-01-01

Background. High throughput DNA sequencing of bulk invertebrate samples or metabarcoding is becoming increasingly used to provide ..

University of Melbourne Researchers

Ary Hoffmann